R Cubeddu et al 2002 J. Phys. D: Appl. Phys. 35 R61 doi:10.1088/0022-3727/35/9/201
R Cubeddu1, D Comelli, C D'Andrea, P Taroni and G Valentini
Show affiliationsFluorescence lifetime imaging is a rather new and effective tool that can be used to study complex biological samples, either at microscopic or macroscopic levels. The map of the fluorescence lifetime allows one to discriminate amongst different fluorophores and to achieve valuable insights into the behaviour of emitting molecules, leading to information like local pH, oxygen concentration in cells, etc. Moreover, the distribution in space of any fluorescent marker achievable with this technique can be exploited for diagnostic purposes in medicine. After a brief introduction on the motivations for applying fluorescence lifetime imaging in biology and medicine, the basic principles of this technique will be addressed. Then, the two possible implementations of fluorescence lifetime imaging (i.e. the frequency domain and the time domain methods) will be presented. For this purpose, special attention will be devoted to practical aspects of image acquisition and processing, especially for what concerns the time domain method. Then, the analysis of the state-of-the-art systems will include a brief discussion on new concepts that have recently been introduced in this research field. Finally, two interesting applications of fluorescence lifetime imaging will be presented. The former refers to skin tumour detection and has been successfully applied in a preliminary clinical trial, the latter regards DNA chips reading and has been tested only at laboratory level, yet it has produced promising results for its future implementation in commercial systems.
Issue 9 (7 May 2002)
Received 12 March 2002
Published 16 April 2002
R Cubeddu et al 2002 J. Phys. D: Appl. Phys. 35 R61
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N Izadi et al 2010 J. Micromech. Microeng. 20 085041
Tianzhun Wu et al 2010 J. Micromech. Microeng. 20 085043
R Parthasarathy 2005 J. Phys. A: Math. Gen. 38 5379
N Straumann 2005 Class. Quantum Grav. 22 2533
R Santra et al 2007 J. Phys.: Conf. Ser. 88 012052
Sylvain Veilleux et al 2009 ApJ 700 L149
P Ingenhoven et al 2009 J. Phys.: Condens. Matter 21 415302
Baris Altunkaynak et al JHEP04(2009)114